Study on inhibitory effect of antisense VEGF RNA on the growth of hepatocellular in vitro in vivo / 中华肝脏病杂志
Chinese Journal of Hepatology
; (12): 725-727, 2003.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-339100
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine the effects of PCMV-FGEV transfection on the profile of SMMC-7721 hepatocellular in vitro in vivo.</p><p><b>METHODS</b>SMMC-7721 hepatocellular was transfected with PCMV-FGEV antisense, PCMV-VEGF sense and empty vector plasmid encapsulated by lipofectamine. The positive cell clones were selected with G418. The stable transfection and expression of VEGF in the SMMC-7721 hepatocellular were determined by in situ hybridization and immunochemical analysis. The effect of PCMV-FGEV transfection on SMMC-7721 hepatocellular proliferation was observed by MTT colorimetric assay. Flow cytometry was used to determine the effects of PCMV-FGEV transfection on cell apoptosis of SMMC-7721. The growth of transfected cells was also observed in nude mice.</p><p><b>RESULTS</b>There was reduced VEGF expression in SMMC-7721 transfected with PCMV-FGEV confirmed by in situ hybridization and immunohistochemical analysis. There was no effect of PCMV-FGEV transfection on cell proliferation and cell apoptosis of SMMC-7721 in vitro. The growth of cell with PCMV-FGEV transfected was slow in nude mice (vivo) and accompanied with obvious apoptosis. The latent time of tumor in the antisense mice group was 25.0+/-1.8 days, which was longer than that in sense and control group significantly (F=19.455, P<0.01). On the other hand, the average tumor weight in antisense group (0.96 g+/-0.28 g) was the smallest among the three groups (F=21.501, P<0.01).</p><p><b>CONCLUSIONS</b>The expression of VEGF was inhibited by PCMV-FGEV. There was no effect on cell proliferation and cell apoptosis of SMMC-7721 by transferring PCMV-FGEV gene into SMMC-7721 cells in vitro. But in vivo it can inhibit tumor growth and induce cell apoptosis.</p>
Full text:
Available
Health context:
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Digestive System Diseases
/
Liver Cancer
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Therapeutics
/
Immunohistochemistry
/
Transfection
/
Cell Division
/
RNA, Antisense
/
Apoptosis
/
Cell Line, Tumor
/
Vascular Endothelial Growth Factor A
/
Therapeutic Uses
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2003
Document type:
Article