A genome sequence of novel SARS-CoV isolates: the genotype, GD-Ins29, leads to a hypothesis of viral transmission in South China / 基因组蛋白质组与生物信息学报·英文版
Genomics, Proteomics & Bioinformatics
; (4): 101-107, 2003.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-339517
Responsible library:
WPRO
ABSTRACT
We report a complete genomic sequence of rare isolates (minor genotype) of the SARS-CoV from SARS patients in Guangdong, China, where the first few cases emerged. The most striking discovery from the isolate is an extra 29-nucleotide sequence located at the nucleotide positions between 27,863 and 27,864 (referred to the complete sequence of BJ01) within an overlapped region composed of BGI-PUP5 (BGI-postulated uncharacterized protein 5) and BGI-PUP6 upstream of the N (nucleocapsid) protein. The discovery of this minor genotype, GD-Ins29, suggests a significant genetic event and differentiates it from the previously reported genotype, the dominant form among all sequenced SARS-CoV isolates. A 17-nt segment of this extra sequence is identical to a segment of the same size in two human mRNA sequences that may interfere with viral replication and transcription in the cytosol of the infected cells. It provides a new avenue for the exploration of the virus-host interaction in viral evolution, host pathogenesis, and vaccine development.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Phylogeny
/
Genetic Variation
/
Molecular Sequence Data
/
Base Sequence
/
Cluster Analysis
/
China
/
Genome, Viral
/
Sequence Analysis, DNA
/
Reverse Transcriptase Polymerase Chain Reaction
/
Severe Acute Respiratory Syndrome
Country/Region as subject:
Asia
Language:
English
Journal:
Genomics, Proteomics & Bioinformatics
Year:
2003
Document type:
Article