Study on the mechanism of how vasonatrin peptide can attenuate the growth-promoting effect of hypoxia in cardiac fibroblasts / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 8-11, 2003.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-339693
Responsible library:
WPRO
ABSTRACT
<p><b>AIM</b>To investigate how vasonatrin peptide (VNP) can attenuate the growth-promoting effect of hypoxia in cardiac fibroblasts cultured from neonatal rats.</p><p><b>METHODS</b>The cultured cardiac fibroblasts were divided randomly into four groups control group, hypoxia group, hypoxia + VNP group and hypoxia + 8-Bromo-cGMP group. The growth of cardiac myocytes was measured by the means of MTT method. The effect of VNP on the intracellular level of cGMP and PCNA were measured by the means of radioimmunoassay and immunohistochemistry stain respectively.</p><p><b>RESULTS</b>Hypoxia (24 h) significantly increased the MTT A490nm value of cardiac fibroblasts (P < 0.05 vs control group). Both VNP (10(-7) mol/L) and 8-Bromo-cGMP (10(-3) mol/L) decreased MTT A490 nm value in cardiac fibroblast (P < 0.05 vs hypoxia group). VNP (10(-7) mol/L) increased the intracellular level of cGMP (P < 0.05 vs control and hypoxia group). Hypoxia (24 h) significantly increased the expression of proliferating cell nuclear antigen (PCNA) in cardiac myocytes (P < 0.05, vs control group), but VNP (10(-7) mol/L) decreased it.</p><p><b>CONCLUSION</b>VNP can attenuate hypoxia-induced growth-promoting effect in cardiac fibroblasts which is associated with the changes of cGMP and PCNA.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Cell Hypoxia
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Cells, Cultured
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Atrial Natriuretic Factor
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Rats, Sprague-Dawley
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Cyclic GMP
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Proliferating Cell Nuclear Antigen
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Cell Biology
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Myoblasts, Cardiac
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Animals, Newborn
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2003
Document type:
Article