Mechanical loading induced expression of bone morphogenetic protein-2, alkaline phosphatase activity, and collagen synthesis in osteoblastic MC3T3-E1 cells / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 4093-4097, 2012.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-339894
Responsible library:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>Bone morphogenetic protein (BMP)-2, alkaline phosphatase (ALP), and collagen type I are known to play a critical role in the process of bone remodeling. However, the relationship between mechanical strain and the expression of BMP-2, ALP, and COL-I in osteoblasts was still unknown. The purpose of this study was to investigate the effects of different magnitudes of mechanical strain on osteoblast morphology and on the expression of BMP-2, ALP, and COL-I.</p><p><b>METHODS</b>Osteoblast-like cells were flexed at four deformation rates (0, 6%, 12%, and 18% elongation). The expression of BMP-2 mRNA, ALP, and COL-I in osteoblast-like cells were determined by real-time quantitative reverse transcription polymerase chain reaction, respectively. The results were subjected to analysis of variance (ANOVA) using SPSS 13.0 statistical software.</p><p><b>RESULTS</b>The cells changed to fusiform and grew in the direction of the applied strain after the mechanical strain was loaded. Expression level of the BMP-2, ALP, and COL-I increased magnitude-dependently with mechanical loading in the experimental groups, and the 12% elongation group had the highest expression (P < 0.05).</p><p><b>CONCLUSION</b>Mechanical strain can induce morphological change and a magnitude-dependent increase in the expression of BMP-2, ALP, and COL-I mRNA in osteoblast-like cells, which might influence bone remodeling in orthodontic treatment.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Osteoblasts
/
Cell Line
/
Analysis of Variance
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Collagen
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Cell Biology
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Collagen Type I
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Alkaline Phosphatase
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Bone Morphogenetic Protein 2
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Metabolism
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2012
Document type:
Article