An immunohistochemical study of the effects of excessive fluoride on type I collagen in rat developmental dentine / 中华劳动卫生职业病杂志
Chinese Journal of Industrial Hygiene and Occupational Diseases
; (12): 429-431, 2003.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-340053
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the effects of excessive fluoride on type I collagen in rat developmental dentine.</p><p><b>METHODS</b>Eighty SD rats, 5 days old, were divided into experimental and control groups, 40 in each group. The experimental group received subcutaneous injection of 0.2% NaF every 4 days (the dose was 2 mg NaF per kg body wt). The same volume of 0.9% NaCl was used in the control. Twenty rats in each group were killed 4 days after the second and the seventh injection respectively. The expression of type I collagen was assayed with immunohistochemical technique.</p><p><b>RESULTS</b>4 out of 20 rats after two injections showed abnormal distribution of type I collagen (dense stain of collagen in the odontoblast, aggregation of collagen in the dentine and disordered arrangement of collagen in the predentine; All 20 rats after seven injections showed abnormal distribution of type I collagen.</p><p><b>CONCLUSION</b>Excessive fluoride may affect the metabolism of type I collagen in rat developmental dentine.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Immunohistochemistry
/
Chemistry
/
Rats, Sprague-Dawley
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Collagen Type I
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Dentin
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Toxicity
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Fluorides
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Animals, Newborn
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Industrial Hygiene and Occupational Diseases
Year:
2003
Document type:
Article