The effects of electromagnetic pulse on fluidity and lipid peroxidation of mitochondrial membrane / 中华劳动卫生职业病杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the effects of intense electromagnetic pulse(EMP) on the biological effects of mitochondrial membrane.</p><p><b>METHOD</b>Rat liver mitochondrial suspension was exposed to EMP at 60 kV/m level. The changes of membrane lipid fluidity and membrane protein mobility were detected by ESR and spin label technique. Malondialdehyde(MDA) was detected by spectrophotometer.</p><p><b>RESULTS</b>The mobility of membrane protein decreased significantly(P < 0.05). Correlation time (tau c) of control group was (0.501 +/- 0.077) x 10(-9)s, and tau c of EMP group was (0.594 +/- 0.049) x 10(-9)s, indicating that the mobility of protein was restricted. The fluidity of mitochondrial membrane increased significantly(P < 0.05) at the same time. Order parameter(S) of mitochondrial membrane lipid in control group was 0.63 +/- 0.01, while S of EMP group was 0.61 +/- 0.01(P < 0.05). MDA decreased significantly.</p><p><b>CONCLUSION</b>The mobility and lipid peroxidation of mitochondrial membrane may be disturbed after EMP exposure.</p>