Molecular mechanism of damage and repair of mouse thymus lymphocytes induced by radiation / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 1070-1073, 2002.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-340384
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of apoptosis in radiation-induced mouse thymus lymphocyte damage and repair and provide the basis for understanding the molecular mechanism of radiation-induced lymphocyte damage and repair as well as the prevention and treatment of acute radiation sickness.</p><p><b>METHODS</b>We studied the dynamic changes of apoptosis of mouse thymus lymphocytes and the expression of bax and bcl-2 gene products after 2, 4, 6 and 8 Gy of whole body gamma-irradiation using in situ terminal labeling, DNA electrophoresis and immunohistochemical techniques.</p><p><b>RESULTS</b>At the early stage after irradiation, the percentage of apoptotic lymphocytes increased rapidly in accordance with the increasing of radiation doses, while the counts of the thymus and peripheral lymphocytes decreased sharply, showing an opposite change to lymphocyte apoptosis. After 6 Gy gamma-irradiation, typical morphological characteristics of thymus apoptotic lymphocytes in early, middle and late stages were found by transmission electron microscopy. The thymus lymphocytes displayed characteristic DNA ladders 4 hr and 8 hr after 2-6 Gy gamma-irradiation,using DNA gel electrophoresis techniques. Abnormal expression of bcl-2 and bax gene products were shown in irradiated lymphocytes.</p><p><b>CONCLUSIONS</b>Apoptosis plays an important role in the process of radiation-induced mouse thymus lymphocyte damage and repair. Bcl-2 and Bax proteins may regulate the process of lymphocyte apoptosis.</p>
Full text:
Available
Health context:
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Endocrine System Diseases
/
Other Malignant Neoplasms
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Radiation Effects
/
Thymus Gland
/
Time Factors
/
Lymphocytes
/
Proto-Oncogene Proteins
/
Apoptosis
/
Proto-Oncogene Proteins c-bcl-2
/
Dose-Response Relationship, Radiation
Limits:
Animals
Language:
English
Journal:
Chinese Medical Journal
Year:
2002
Document type:
Article