Expression of brain-derived neurotrophic factor at acute inflammatory injury of the brain / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 433-436, 2003.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-341981
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of brain-derived neurotrophic factor (BDNF) mRNA and immunoreactivity in experimental acute inflammatory brain injury.</p><p><b>METHODS</b>Ten rats were inoculated with pneumococcus to establish the model of bacterial inflammatory brain injury and other 6 rats were used as normal controls. At 24 h after inoculating, the expression of BDNF mRNA and BDNF protein in brain tissue was detected by in situ hybridization and immunohistochemical methods, respectively.</p><p><b>RESULT</b>The necrosis of neuron in cerebral cortex and hippocampus was observed after infection. The increase of BDNF mRNA expression in the cerebral cortex and hippocampus of experimental animals was demonstrated at 24 h after inoculation (0.1194 +/- 0.02941 compared with 0.0662 +/- 0.01176)A and (0.1608 +/-0.01854 compared with 0.0680 +/- 0.00946)A (P<0.01), respectively. Compared with controls the expression of BDNF protein in the cerebral cortex and hippocampus was enhanced at 24 h of inoculation(177.04+/-43.66 compared with 79.79+/-7.23)mm(2) (P<0.01) and (81.78 +/-37.47 compared with 42.98 +/-20.44)mm(2) (P<0.01), respectively. Strong positive hybridization and immunoreactivity were observed in the infiltrated inflammatory cell in leptomeninges, subarachnoid cavity, ventricles and brain parenchyma in the brain from the experimental rats.</p><p><b>CONCLUSION</b>The expression of BDNF mRNA and BDNF protein increases following brain inflammatory injury, which supports the hypothesis that BDNF may constitute intrinsic neuroprotective mechanism as a part of the inflammatory response.</p>
Full text:
Available
Health context:
Neglected Diseases
/
SDG3 - Target 3.3 End transmission of communicable diseases
Health problem:
Zoonoses
/
Meningitis
Database:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Immunohistochemistry
/
Calcium
/
Acute Disease
/
Rats, Sprague-Dawley
/
Brain-Derived Neurotrophic Factor
/
Genetics
/
Meningitis, Pneumococcal
/
Metabolism
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2003
Document type:
Article