Purification of enramycin by macroporous resin adsorption and reversed phase chromatography purification / 生物工程学报
Chinese Journal of Biotechnology
; (12): 1701-1708, 2014.
Article
in Zh
| WPRIM
| ID: wpr-345553
Responsible library:
WPRO
ABSTRACT
Enramycin is a polypeptide antibiotic and new, safe animal feed additive. A new purification process was developed, based on pre-purification by macroporous resin and refining by reversed phase chromatography. AB-8 macroporous resin was used for the pre-purification process of enramycin, with an elution buffer of 0.012 mol/L aqueous HCl solution-methanol (50: 50, V/V). Then, enramycin a and enramycin b were separated effectively by C18 reversed phase chromatography, with a elution buffer of 0.05 mol/L aqueous KH2PO4 solution-acetonitrile (70: 30, V/V, pH 4.5). The purities of enramycin a and enramycin b were up to 98.5% and 98.0%, respectively. The yield reached 29.2%. This study would provide a useful reference for the preparation of enramycin a and enramycin b with a high purity.
Full text:
1
Database:
WPRIM
Main subject:
Peptides
/
Adsorption
/
Chromatography, Reverse-Phase
/
Methods
/
Anti-Bacterial Agents
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2014
Document type:
Article