Study of hypermethylation of SOCS gene in typical myeloproliferative disease / 中华血液学杂志
Chinese Journal of Hematology
; (12): 772-776, 2011.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-345993
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the clinical role of hypermethylation of suppressor of cytokine signaling (SOCS) on typical myeloproliferative disease (MPD) patients and its mechanism.</p><p><b>METHODS</b>Methylation specific PCR was used to detect SOCS1, 2, 3 methylation, direct DNA sequencing was performed to detect JAK2V617F mutation, real-time fluorescence quantitative PCR were applied to evaluate transcriptional activity of SOCS1, 2, 3.</p><p><b>RESULTS</b>Among 100 MPD patients, hypermethylation of SOCS1 was detected in 27 (27%), hypermethylation of SOCS2 in 9 (9%), hypermethylation of SOCS3 in 34 (34%); JAK2V617F mutation in 64 (64%). Hypermethylation of SOCS1, 3 greatly inhibited gene expression compared with unmethylated ones (P < 0.05). Presence of JAK2V617F mutation markedly down-regulated SOCS1, 3 gene mRNA expression compared with wild JAK2V617F (P < 0.05).</p><p><b>CONCLUSION</b>Hypermethylation of SOCS1, 3 and JAK2V617F mutation exist in MPD, which inhibited SOCS1, 3 gene expression. SOCS hypermethylation and JAK2V617F mutation can activate JAK-STAT signaling pathways, these observations may provide a potential therapeutic direction.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
RNA, Messenger
/
Signal Transduction
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DNA Methylation
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Suppressor of Cytokine Signaling Proteins
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Janus Kinase 2
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Genetics
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Metabolism
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Mutation
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Myeloproliferative Disorders
Limits:
Adolescent
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Adult
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Aged
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Aged, 80 and over
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Female
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Humans
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Male
Language:
Chinese
Journal:
Chinese Journal of Hematology
Year:
2011
Document type:
Article