Study on in vitro/in vivo correlation of extracts from Ligusticum chuanxiong after transdermal administration / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 422-426, 2013.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-346804
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the transdermal permeation and in vivo pharmacokinetics of effective constituent ferulic acid from Ligusticum chuanxiong, in order to establish the in vitro/in vivo correlation in transdermal permeation.</p><p><b>METHOD</b>Franz diffusion cell was adopted in the in vitro transdermal permeation, with CD-1 nude rat abdominal skin as the permeation medium. Linear probes were implanted in CD-1 nude rats. With PBS as perfusate, microdialysis was employed to study the pharmacokinetics. Ferulic acid concentrations in the receptor solution and dialysate were assessed by high performance liquid chromatography (HPLC). The study on correlation between in vitro and in vivo data was conducted by deconvolution methods.</p><p><b>RESULT</b>The transdermal permeation rate of ferulic acid from Ligusticum chuanxiong was (0.094 4 +/- 0.049 4) microg x cm2 x min, with Cmax of ferulic acid being 808.91 microg x L(-1), and Tmax being 183 min after dermal administration. The in vitro/in vivo correlation was 93.61.</p><p><b>CONCLUSION</b>Ferulic acid in extracts from L. chuanxiong can quickly penetrate skins. By using the in vitro/in vivo correlation in transdermal permeation, simple in vitro transdermal permeation method can be adopted to study the changes in its pharmacokinetics.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Permeability
/
Skin
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Skin Absorption
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In Vitro Techniques
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Administration, Cutaneous
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Rats, Nude
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Drugs, Chinese Herbal
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Pharmacokinetics
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Metabolic Clearance Rate
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Chromatography, High Pressure Liquid
Limits:
Animals
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2013
Document type:
Article