Induction of adventitious roots of Echinacea pallida and accumulation of caffeic acid derivatives / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 3768-3772, 2012.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-346841
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of auxins 2,4-D,IAA,IBA,NAA on induction of adventitious roots as well as that of IBA concentrations on the growth of adventitious roots and the accumulation of caffeic acid derivatives, with test-tube seedling leaves Echinacea pallida as the explant, and cultivate adventitious roots in bioreactors.</p><p><b>RESULT</b>1.0 mg x L(-1) IBA was found the best for the induction of adventitious roots, with the numer of induced adventitious roots up to 22. 5 in each culture dish. Among different concentrations for suspension cultivation of IBA tested, 1.0 mg x L(-1) IBA was found the most suitable for the growth of adventitious roots and the accumulation of caffeic acid derivatives. In a 5 L balloon type bubble bioreactor, 8.98 g x L(-1) dry weight was achieved after one month, which was 2.05 times of 4.38 g x L(-1) dry weight cultivated in a triangular flask. The content of echinacoside cultivated in a bioreactor was 14.08 mg x g(-1) DW, which was 2.4 times of cultivated roots. The contents of chlorogenic acid, chicoric acid and total caffeic acid derivatives were 4.0-25.6 times of ultivated roots.</p><p><b>CONCLUSION</b>The study can provide high-quality biomedical drugs containing such caffeic acid derivatives as echinacoside for mass production of Echinacea purpurea medicines.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Plant Growth Regulators
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Caffeic Acids
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Chemistry
/
Plant Roots
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Plant Leaves
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Bioreactors
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Echinacea
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Seedlings
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Tissue Culture Techniques
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2012
Document type:
Article