Extract and identify ingredient from Ligustrum Lucidum Ait and study its effect to periodontal pathogen / 中华口腔医学杂志
Chinese Journal of Stomatology
; (12): 388-390, 2002.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-347366
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To extract the effective ingredient (crystal I) from effective section (saponin) of Ligustrum Lucidum Ait, identify the chemical structure of crystal I, study the effect of crystal I on P. gingivalis, B. forsythus and P. intermedia.</p><p><b>METHODS</b>Isolated crystal I from saponin using the silica gel column chromatograph. Identified crystal I with IR spectra, (1)H-NMR and (13)C-NMR. Measured the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) through micro-liquid dilution. Studied the killing curve of ursolic acid on B. forsythus and P. intermedia.</p><p><b>RESULTS</b>The crystal I was identified as ursolic acid; its MIC and MBC to P. gingivalis, B. forsythus and P. intermedia were 0.740 and 0.295 microg/L respectively. The killing curve indicated that 0.800 microg/L ursolic acid could kill P. intermedia and B. forsythus in 3 and 6 hours respectively.</p><p><b>CONCLUSION</b>Ursolic acid has obvious effect to inhibit periodontal pathogen.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Bacteroides
/
Triterpenes
/
Periodontium
/
Plant Extracts
/
Magnetic Resonance Spectroscopy
/
Microbial Sensitivity Tests
/
Cell Division
/
Porphyromonas gingivalis
/
Prevotella intermedia
Language:
English
Journal:
Chinese Journal of Stomatology
Year:
2002
Document type:
Article