Value of diagnosing micrometastasis by nested RT-PCR in the peripheral blood and bone marrow in non-small cell lung cancer patients / 中华肿瘤杂志
Chinese Journal of Oncology
; (12): 62-65, 2003.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-347492
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To evaluate the specificity, sensitivity and their clinical significance of detecting CK19 mRNA expression by nested RT-PCR for molecular diagnosis of micrometastasis in the peripheral blood and bone marrow of patients with non-small cell lung cancer.</p><p><b>METHODS</b>CK19 mRNA expression was detected by nested RT-PCR in peripheral blood and bone marrow samples from 59 lung cancer patients, with samples of 11 benign pulmonary lesion patients and 20 healthy adults as control.</p><p><b>RESULTS</b>The sensitivity of nested RT-PCR was 10(-6). The positive rates of micrometastasis were 33.89% (20/59) in peripheral blood and 22.03% (13/59) in bone marrow, with a highly positive correlation existing between the two groups (P < 0.05). The micrometastasis in peripheral blood and bone marrow was closely correlated with the pathological classification and cell differentiation (P < 0.05) and P-TNM stage (P < 0.01). No CK19 mRNA expression was found in the samples from patients with benign pulmonary lesion or healthy adult volunteers.</p><p><b>CONCLUSION</b>The peripheral blood and bone marrow from patients with non-small cell lung cancer possesses micrometastasis that can not be detected by common methods. Nested RT-PCR technique shows favorable specificity and sensitivity in detecting the condition with definite clinical prospects.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Bone Marrow
/
Biomarkers, Tumor
/
Sensitivity and Specificity
/
Carcinoma, Non-Small-Cell Lung
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Reverse Transcriptase Polymerase Chain Reaction
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Lung Neoplasms
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Methods
/
Neoplasm Metastasis
Type of study:
Diagnostic study
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Oncology
Year:
2003
Document type:
Article