Residual collagen binding assay for von Willebrand factor-cleaving protease activity and its clinical application / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 726-729, 2004.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-347875
Responsible library:
WPRO
ABSTRACT
von Willebrand factor-cleaving protease (vWF-cp) is a newly identified metalloproteinase. The activity of vWF-cp would vary in different physiological or pathological states. To explore activity detectron of von Willebrand factor-cleaving protease and its clinical application, the vWF-cp activity was measured by a sensitive enzyme-linked immunoadsorbent assay to detect the residual collagen binding activity (R-CBA) of von Willebrand factor before and after digestion with vWF-cp. Moreover, its activity deficiency in patients with thrombotic thrombocytopenic purpura (TTP) and solid tumors was also investigated. The results showed that the residual collagen binding assay was sensitive enough to measure the serum or plasma vWF-cp activity in 87 health individuals, 79 patients suffering from TTP and solid tumors. The coefficient of variation within and between the batches was were 3.60% and 8.35%, respectively. The serum and plasma vWF-cp activity in health individuals was (79.47 +/- 10.78)% (n = 53) and (78.79 +/- 9.17)% (n = 30), respectively, whereas the vWF-cp activity in patients with TTP, benign and malignant tumors was significantly decreased (P values were less than 0.001, 0.03 and 0.001, respectively). It is concluded that the vWF-cp activity in plasma or serum of patients with TTP and solid tumors markedly decrease, especially in patients with TTP. Assay of the vWF-cp activity using R-CBA is a simple method.
Full text:
Available
Health context:
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Digestive System Diseases
/
Other Hemoglobinopathies and Hemolytic Anaemias
/
Other Blood Disorders
/
Stomach Cancer
Database:
WPRIM (Western Pacific)
Main subject:
Purpura, Thrombotic Thrombocytopenic
/
Stomach Neoplasms
/
Blood
/
Von Willebrand Factor
/
Enzyme-Linked Immunosorbent Assay
/
Diagnosis
/
ADAM Proteins
/
ADAMTS13 Protein
/
Metabolism
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Adolescent
/
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2004
Document type:
Article