Microbial glycosylation of cardamonin by Mucor spinosus / 药学学报
Acta Pharmaceutica Sinica
; (12): 733-737, 2011.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-348892
Responsible library:
WPRO
ABSTRACT
Microbial transformation of cardamonin by Mucor spinosus (CGMCC 3.3450) in preparative scale resulted in the isolation of two new products. Their structures were elucidated unambiguously by ESI-MS, 1H NMR, 13C NMR and 2D NMR spectra analyses as 4-O-beta-D-glucopyranosyl-6-hydroxy-2-methoxychalcone (1, 4-GluC) and 6-O-beta-D-glucopyranosyl-4-hydroxy-2-methoxychalcone (2, 6-GluC), respectively. The time-course of biotransformation by M. spinosus showed that both 4-GluC and 6-GluC appeared on the 2nd day. The optimal biotransformation temperature was 28 degrees C, the optimal biotransformation time was 72 h and the optimal concentration for cardamonin was 40 mg x mL(-1). This is the first time for successful microbial glycosylation of cardamonin in present research.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Glycosylation
/
Biotransformation
/
Magnetic Resonance Spectroscopy
/
Molecular Structure
/
Chemistry
/
Spectrometry, Mass, Electrospray Ionization
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Chalcones
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Glucosides
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Metabolism
/
Mucor
Language:
English
Journal:
Acta Pharmaceutica Sinica
Year:
2011
Document type:
Article