Study on the correlation of GCS and MDR1 genes in inducing multidrug resistance in human K562/A02 cell line / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 299-304, 2010.
Article
in Zh
| WPRIM
| ID: wpr-348992
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the correlation of glucosylceramide synthase (GCS) gene and multidrug resistance 1 (MDR1) gene in inducing multidrug resistance in human multidrug-resistant K562/A02 cell line, and search for a novel strategy for reversing multidrug resistance of leukemia cells.</p><p><b>METHODS</b>The expression levels of GCS and MDR1 mRNA in K562 and K562/A02 cells were assayed by RT-PCR. siRNAs targeting the GCS and MDR1 gene were transfected into K562/A02 cells with liposome, respectively. The differential expression of GCS and MDR1 mRNAs, as well as their correlation, were detected by RT-PCR and real time quantitative-PCR(QPCR).</p><p><b>RESULTS</b>The expression level of GCS and MDR1 mRNA was dramatically lower in drug-sensitive K562 cells compared with the K562/A02 cells. The GCS mRNA was inhibited by 73%(59%-82%) and MDR1 mRNA expression was down regulated by 67% (38%-82%) in K562/A02 cells after being transfected with GCS siRNA. The expression level of MDR1 mRNA was inhibited by 81%(63%-91%) and GCS mRNA expression had no apparent change in K562/A02 cells treated with MDR1 small interference RNA(siRNA).</p><p><b>CONCLUSION</b>Positive correlation was detected between the expression of GCS and MDR1 mRNA in K562/A02 cells and MDR1 mRNA expression was down regulated after silencing the GCS gene expression.</p>
Full text:
1
Database:
WPRIM
Main subject:
Physiology
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RNA, Messenger
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ATP Binding Cassette Transporter, Subfamily B, Member 1
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Drug Resistance, Multiple
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Drug Resistance, Neoplasm
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Reverse Transcriptase Polymerase Chain Reaction
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RNA, Small Interfering
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Cell Line, Tumor
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Genetics
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Glucosyltransferases
Limits:
Humans
Language:
Zh
Journal:
Chinese Journal of Medical Genetics
Year:
2010
Document type:
Article