Low programmed cell death 5 expression is a prognostic factor in ovarian cancer / 中华医学杂志(英文版)
Chinese Medical Journal
; (24): 1084-1090, 2015.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-350347
Responsible library:
WPRO
ABSTRACT
<p><b>BACKGROUND</b>Ovarian cancer is a leading gynecological malignancy. We investigated the prognostic value of programmed cell death 5 (PDCD5) in patients with ovarian cancer.</p><p><b>METHODS</b>Expression levels of PDCD5 mRNA and protein were examined in six ovarian cancer cell lines (SKOV3, CAOV3, ES2, OV1, 3AO, and HOC1A) and one normal ovarian epithelial cell line (T29) using reverse transcription polymerase chain reaction, Western blotting, and flow cytometry. After inducing PDCD5 induction in SKOV3 cells or treating this cell line with taxol or doxorubicin (either alone or combined), apoptosis was measured by Annexin V-FITC/propidium iodide staining. Correlations between PDCD5 protein expression and pathological features, histological grade, FIGO stage, effective cytoreductive surgery, and serum cancer antigen-125 values were evaluated in patients with ovarian cancer.</p><p><b>RESULTS</b>PDCD5 mRNA and protein expression were downregulated in ovarian cancer cells. Recombinant human PDCD5 increased doxorubicin-induced apoptosis in SKOV3 cells (15.96 ± 2.07%, vs. 3.17 ± 1.45% in controls). In patients with ovarian cancer, PDCD5 expression was inversely correlated with FIGO stage, pathological grade, and patient survival (P < 0.05, R = 0.7139 for survival).</p><p><b>CONCLUSIONS</b>PDCD5 expression is negatively correlated with disease progression and stage in ovarian cancer. Therefore, measuring PDCD5 expression may be a good method of determining the prognosis of ovarian cancer patients.</p>
Full text:
Available
Health context:
SDG3 - Target 3.4 Reduce premature mortality due to noncommunicable diseases
Health problem:
Endocrine System Diseases
/
Ovary Cancer
Database:
WPRIM (Western Pacific)
Main subject:
Ovarian Neoplasms
/
Pathology
/
Prognosis
/
In Vitro Techniques
/
Cell Line, Tumor
/
Apoptosis Regulatory Proteins
/
Genetics
/
Metabolism
/
Neoplasm Proteins
Type of study:
Prognostic study
Limits:
Adult
/
Aged
/
Female
/
Humans
Language:
English
Journal:
Chinese Medical Journal
Year:
2015
Document type:
Article