A novel trichostatin analogue culture of Streptomyces sp. CPCC 203909 / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1751-1754, 2015.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-351271
Responsible library:
WPRO
ABSTRACT
By using a cell-based high throughput screening model for the CLA-1 up-regulator, Streptomyces 203909 was found to produce up-regulator of CLA-1. A novel trichostatin analogue was isolated from the rice fermentation of Streptomyces sp. CPCC 203909by a combination of various chromatographic techniques including column chromatography (CC) over silica gel, flash C18 CC, and reversed-phase HPLC. Its structure was identified as (-)-(R,2E,4Z)-7-[(4'-dimethylamino) phenyl]-4,6-dimethyl-7-oxohepta-2,4-dienoyl-L-glutamine (1) by the spectroscopic and chemical methods, and combination with the CD spectroscopy and Marfey's method. In the prelimi- nary assays, Compound 1 showed cytotoxicity against human embryonic kidney 293 cell line with IC50 value 35.3 [µmol · L(-1).
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Streptomyces
/
Molecular Structure
/
Cell Survival
/
Chemistry
/
Hep G2 Cells
/
Fermentation
/
Hydroxamic Acids
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2015
Document type:
Article