Regulatory effect of coptisine on key genes involved in cholesterol metabolism / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 1548-1553, 2015.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-351310
Responsible library:
WPRO
ABSTRACT
To study the effect of cholesterol and 25-OH-cholesterol on cholesterol metabolism in HepG2 cells and the effect of coptisine (Cop) extracted from Coptidis Rhizoma (CR) in reducing and regulating cholesterol. In this study, TC, TG, LDL-c and HDL-c were measured by biochemical analysis; mRNA and protein expressions of LDLR, HMGCR and CYP7A1 were detected by qRT-PCR and Western blot. According to the results, cholesterol and 25-OH-cholesterol inducing could decrease in mRNA and protein expressions of LDLR and CYP7A1, so as to increase TC and LDL-c contents. However, Cop could up-regulate mRNA and protein expressions of LDLR and CYP7A1 and down-regulate that of HMGCR, so as to reduce TC and LDL-c levels. These findings suggested that Cop has potential pharmacological activity for reducing cholesterol, and may reduce cholesterol by regulating mRNA and protein expressions of key genes involved in cholesterol metabolism, such as LDLR, CYP7A1 and HMGCR. This study laid a firm theoretical foundation for developing new natural drugs with the cholesterol-lowering activity.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Triglycerides
/
Berberine
/
Receptors, LDL
/
Drugs, Chinese Herbal
/
Cholesterol 7-alpha-Hydroxylase
/
Gene Expression Regulation, Enzymologic
/
Cholesterol
/
Hep G2 Cells
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
China Journal of Chinese Materia Medica
Year:
2015
Document type:
Article