Expression of porcine interleukin-18 in baculovirus/insect cells / 生物工程学报
Chinese Journal of Biotechnology
; (12): 118-123, 2011.
Article
in Zh
| WPRIM
| ID: wpr-351527
Responsible library:
WPRO
ABSTRACT
IL-18, as a polyphonic cytokine, is important in immune response and physiologic function. We designed one pair of primers, amplified the porcine IL-18 gene fused with a C-terminal 6xHistidine tag, and then subcloned into the pFastBacDual of Baculovirus transfer vector and transformed into DH10Bac containing a shuttle vector of Bacmid. After co-transfecting the recombinant plasmid into insect cells, the 18 kDa expressed protein of porcine IL-18 was detected by SDS-PAGE; the specificity of expressed protein was confirmed by Western blotting. The purified porcine IL-18 protein induced obvious proliferation of porcine T lymphocytes in vitro, which indicated that the expression of IL-18 had high biological activity.
Full text:
1
Database:
WPRIM
Main subject:
Swine
/
Recombinant Proteins
/
Transfection
/
Cells, Cultured
/
Baculoviridae
/
Interleukin-18
/
Allergy and Immunology
/
Genetic Vectors
/
Genetics
/
Insecta
Limits:
Animals
Language:
Zh
Journal:
Chinese Journal of Biotechnology
Year:
2011
Document type:
Article