In vitro absorption mechanism of strychnine and the transport interaction with liquiritin in Caco-2 cell monolayer model / 药学学报
Acta Pharmaceutica Sinica
; (12): 1160-1164, 2010.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-353407
Responsible library:
WPRO
ABSTRACT
To study the effect of liquiritin (Liq) on the transport of strychnine (Str) in Caco-2 cell monolayer model, the transport parameters of Str, such as apparent permeability coefficient (P app (B-->A) and P app (A-->B)) and cumulative transport amount (TRcum), were determined and comparatively analyzed when Str was used solely and co-used with Liq. The effect of drug concentrations, conveying times, P-glycoprotein (P-gp) inhibitor verapamil and conveying liquor pH values on the transport of Str were also investigated. The results indicated that the absorption of Str in Caco-2 cell monolayer model was well and the passive transference was the main intestinal absorption mechanism of Str in the Caco-2 monolayer model, along with the excretion action mediated by P-gp. Liq enhanced the absorption of Str. Meanwhile, conveying liquor pH value had significant influence on the excretion transport of Str.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Permeability
/
Pharmacology
/
Plants, Medicinal
/
Strychnine
/
Biological Transport
/
Pharmacokinetics
/
Verapamil
/
Chemistry
/
ATP Binding Cassette Transporter, Subfamily B, Member 1
/
Caco-2 Cells
Limits:
Humans
Language:
Chinese
Journal:
Acta Pharmaceutica Sinica
Year:
2010
Document type:
Article