Preparation of cardiomyocyte-targeting liposomes and their targeting ability in vitro / 药学学报

ABSTRACT

<p><b>AIM</b>To prepare the cardiomyocyte-targeting liposomes and investigate their cardiomyocyte targetability in vitro.</p><p><b>METHODS</b>Liposomes modified with compound PAC were prepared (PAC-L); The uptake of PAC-L by cardiomyocytes was studied by incubating fluorescence labeled liposomes with cardiomyocytes in vitro and measuring the association of liposomes by a fluorescence spectrophotometer.</p><p><b>RESULTS</b>A high affinity of PAC-L to the cardiomyocytes was observed, the amount of cell uptake of PAC-L by cardiomyocytes was higher than that by nonmyocyte (P < 0.001); The amount of cardiomyocyte uptake of PAC-L on the normoxia condition 2 h was 2.9-fold higher than that of plain-L, and the increase was 5.2-fold when hypoxia occured; The form of liposome uptake changed, the amount of cardiomyocyte uptake of Plain-L by internalization was only 11%, while that of PAC-L was 56%.</p><p><b>CONCLUSION</b>It is indicated that PAC-L was a potential drug carrier for targeting to ischemic myocardium.</p>