Tumor-targeted human telomerase reverse transcriptase promoter/tk gene therapy against human nasopharyngeal carcinoma cells in vitro / 南方医科大学学报
Journal of Southern Medical University
; (12): 695-699, 2010.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-355041
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the targeted killing effect of human telomerase reverse transcriptase promoter (hTERTp)/tk gene on human nasopharyngeal carcinoma (NPC) cells.</p><p><b>METHODS</b>The recombinant plasmid hTERTp/tk/pGL3 was transfected into human NPC HNE1 cells and the expressions of TK and telomerase were investigated. The targeted killing effect induced by hTERTp/tk on HNE1 cells was assessed using RT-PCR and MTT assay.</p><p><b>RESULTS</b>TK gene expression was detected in HNE1 cells transfected by hTERTp/tk/pGL3, and the cells showed reduced telomerase and hTERT expression as compared with the control cells. hTERTp/tk/pGL3 resulted in target killing of HNE1 cells but not of the normal control cells. The tumor cell-killing effect of hTERTp/tk/pGL3 was slightly milder than that of the positive control CMV/tk/pGL3 that produced nonselective cell killing.</p><p><b>CONCLUSION</b>hTERTp/tk, a tumor-specific expression system, allows targeted tumor cell killing and reduces the activity of telomerase in NPC cells in vitro.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Thymidine Kinase
/
Transfection
/
Genetic Therapy
/
Nasopharyngeal Neoplasms
/
Promoter Regions, Genetic
/
Gene Targeting
/
Telomerase
/
Cell Line, Tumor
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Journal of Southern Medical University
Year:
2010
Document type:
Article