Cytotoxic effect of cisplatin with proteasome inhibitor on osteosarcoma cells / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 395-399, 2005.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-355196
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To observe the cytotoxic effect of cisplatin with proteasome inhibitor on osteosarcoma cells.</p><p><b>METHODS</b>Cell survival was tested by MTT, apoptotic morphology was observed by electron microscopy, apoptotic rate was analyzed by flow cytometry, the transcription level of excision repair cross complementation-1 (ERCC-1) was tested by reverse transcription polymerase reaction.</p><p><b>RESULTS</b>Compared with cells treated with cisplatin alone, cells treated with cisplatin and proteasome inhibitor showed a decreased survival rate, more typical apoptotic morphology, higher apoptotic rate [(14.37 +/-2.37)% vs. (50.93 +/-4.84)%, P<0.01)], and lower transcription level of excision repair cross complementation-1.</p><p><b>CONCLUSION</b>Proteasome inhibitor could increase the cytotoxic effect of cisplatin on osteosarcoma cells and promote cisplatin-induced osteosarcoma apoptosis. These effects may be associated with the decreased transcription of excision repair cross complementation-1.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Oligopeptides
/
Pathology
/
Pharmacology
/
Bone Neoplasms
/
Tumor Cells, Cultured
/
Osteosarcoma
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Cisplatin
/
Apoptosis
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Drug Synergism
/
Antineoplastic Agents
Limits:
Animals
Language:
Chinese
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2005
Document type:
Article