Lentivirus-mediated angiotensin-converting enzyme 2 overexpression inhibits angiotensin II-induced albumin down-regulation and enhancement of migration in rat hepatocytes / 南方医科大学学报
Journal of Southern Medical University
; (12): 652-658, 2015.
Article
in Zh
| WPRIM
| ID: wpr-355309
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the inhibitory effect of angiotensin-converting enzyme 2 (ACE2) on angiotensin II (Ang II)-induced down-regulation of albumin expression and enhancement of cell migration in rat hepatocytes.</p><p><b>METHODS</b>Cultured rat hepatocyte were treated with Ang II (10-7 mol/L) for different time lengths, and the protein expressions of vimentin and albumin and cell migration were detected. The cells transfected with lentiGFP or lentiACE2 were treated with A779 for 1 h and then with Ang II, and Western blotting and immunofluorescent cytochemistry were used to detect the protein levels; the cell migration was evaluated by Transwell assay.</p><p><b>RESULT</b>Ang II induced significantly increased vimentin expression and reduced albumin expression in rat hepatocytes in a time-dependent manner. Overexpression of ACE2 obviously inhibited the up-regulation of vimentin expression, reduction of albumin expression, and enhancement of cell migration induced by Ang II.</p><p><b>CONCLUSION</b>ACE2 overexpression can inhibit Ang II-induced up-regulation of vimentin, reduction of albumin expression, and enhancement of cell migration in rat hepatocytes.</p>
Full text:
1
Database:
WPRIM
Main subject:
Pharmacology
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Vimentin
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Angiotensin II
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Transfection
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Down-Regulation
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Up-Regulation
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Cell Movement
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Cells, Cultured
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Peptidyl-Dipeptidase A
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Lentivirus
Limits:
Animals
Language:
Zh
Journal:
Journal of Southern Medical University
Year:
2015
Document type:
Article