Effect of angiotensin II on cord blood CD34(+) cells expansion in vitro / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 227-229, 2003.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-355677
Responsible library:
WPRO
ABSTRACT
Angiotensin II (Ang II) is one active substance of renin-angiotensin system. In order to explore the effect of Ang II combined with various cytokines on proliferation and differentiation of cord blood CD34(+) cells, in vitro experiments of cell cultures of Ang II with or without cytokines were taken place. The results showed that Ang II stimulated both BFU-E and CFU-GM expansion. The numbers of BFU-E and CFU-GM raised with increase of Ang II concentrations ranged from 0.01 - 0.1 micro mol/L. In semi-solid culture assay, Ang II stimulated CFU-GM production but no effect on BFU-E occurred. The multiple number of CFU-GM increased from 2.3 +/- 0.8 to 7.8 +/- 1.9 times when Ang II was added into SCF + G-CSF + GM-CSF + IL-3 combination. Similarly, the multiple number of BFU-E increased from 3.1 +/- 1.8 to 9.2 +/- 2.3 times when Ang II was combined with SCF + EPO + TPO + IL-3. In conclusion, Ang II stimulated cord blood hematopoietic stem/progenitor cell expansion in vitro the in presence of various cytokines.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Thrombopoietin
/
Blood
/
Angiotensin II
/
Hematopoietic Stem Cells
/
Erythroid Precursor Cells
/
Cell Differentiation
/
Cell Division
/
Cells, Cultured
/
Granulocyte Colony-Stimulating Factor
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2003
Document type:
Article