Plant regeneration of Withania somnifera / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study tissue culture and plant regeneration of Withania somnifera.</p><p><b>METHOD</b>Leaves of W. somnifera were used for explants, effects of different plant growth substances on callus and shoot induction were studied, different medium and plant growth substances for rooting induction was optimized.</p><p><b>RESULT AND CONCLUSION</b>The best plant growth substances combination for callus induction was MS + 1.0 mg x L(-1) 2,4-D + 0.1 mg x L(-1) KT. The optimal medium for germination was MS + 1.0 mg x L(-1) 6-BA + 0.1 mg x L(-1) NAA. The best medium and plant growth substances combination for rooting induction was 1/2MS + 0.5 mg x L(-1) NAA, transplant survival rate of plantlet reached 92% in humus soil-pearlite (1:1).</p>