Effects of human HSF1 on transcriptional regulation of prohibitin promoter / 中国应用生理学杂志
Chinese Journal of Applied Physiology
; (6): 349-352, 2009.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-356256
Responsible library:
WPRO
ABSTRACT
<p><b>AIM</b>To assay the transcriptional regulation effect of hHSF1 on prohibitin gene promoter.</p><p><b>METHODS</b>The total length of hHSF1 exon was amplified by PCR method and cloned into pcDNA3. 1(+) vector. pcDNA3. 1(+)-hHSF1 and pGI3 prohibitin were co-transfected into HEK293 cells. The luciferase activity was detected by Dual-Luciferase Reporter Assay System to evaluate the transcriptional regulation effect of hHSF1 on prohibitin gene promoter.</p><p><b>RESULTS</b>The pcDNA3.1(+)-hHSF1 vector was constructed successfully. The assay of luciferase activity showed that the transcription of pGL3-prohibitin was dramatically upregulated by hHSF1.</p><p><b>CONCLUSION</b>hHSF1 can transcriptionally regulate prohibitin expression.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Repressor Proteins
/
Transcription Factors
/
Transcription, Genetic
/
Molecular Sequence Data
/
Base Sequence
/
Transfection
/
Gene Expression Regulation
/
Promoter Regions, Genetic
/
DNA-Binding Proteins
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Applied Physiology
Year:
2009
Document type:
Article