Toll-like receptor 4 promotes macrophage foam cell formation induced by oxidized low-density/β₂-glycoprotein I/β₂-glycoprotein I antibodies complex / 中华心血管病杂志
Chinese Journal of Cardiology
; (12): 208-213, 2014.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-356408
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To explore the role of toll-like receptor 4 (TLR4) on oxidized low-density/β₂-glycoprotein I/β₂-glycoprotein I (ox-LDL/β₂GPI/anti-β₂GPI) antibodies complex induced macrophage foam cell formation.</p><p><b>METHODS</b>The peritoneal macrophages were separated from TLR4 intact C3H/HeN mice and TLR4 defective C3H/HeJ mice. The cells were treated with ox-LDL, ox-LDL/β₂GPI, ox-LDL/anti-β₂GPI, anti-β₂GPI/β₂GPI, ox-LDL/β₂GPI/anti-β₂GPI, lipopolysaccharide (LPS) for 48 h and the foam cells were identified by Oil red O staining for intracellular lipids. The total cellular RNA and the protein lysates were collected. The levels of tissue factor (TF) mRNA in two groups were detected by real-time PCR (RT-PCR), and the expression of phosphorylated nuclear factor-κB (NF-κB) p65 was detected by Western blotting. Monocyte chemotactic protein-1 (MCP-1) secretion from peritoneal macrophages was determined by enzyme linked immunosorbent assay (ELISA) kits.</p><p><b>RESULTS</b>Compared with C3H/HeJ mice, lipid droplets in the cytoplasm of peritoneal macrophages from C3H/HeN mice were significantly increased and phosphorylation-NF-κB expression was significantly upregulated after stimulating by ox-LDL/β₂GPI/anti-β₂GPI complex (P < 0.01). TF mRNA and MCP-1 expression were also upregulated post ox-LDL/β₂GPI/anti-β₂GPI complex stimulation [TF mRNA 0.041 ± 0.023 vs. 0.005 ± 0.003; MCP-1 (6 200.2 ± 6.4) pg/ml vs. (803.3 ± 5.5) pg/ml, P < 0.01].</p><p><b>CONCLUSION</b>TLR4 can enhance ox-LDL/β₂GPI/anti-β₂GPI complex induced peritoneal macrophage foam cell formation via upregulating phosphorylation-NF-κB, TF and MCP-1 expression.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Thromboplastin
/
Cells, Cultured
/
NF-kappa B
/
Macrophages, Peritoneal
/
Allergy and Immunology
/
Atherosclerosis
/
Toll-Like Receptor 4
/
Beta 2-Glycoprotein I
/
Foam Cells
/
Lipoproteins, LDL
Type of study:
Prognostic study
Limits:
Animals
Language:
Chinese
Journal:
Chinese Journal of Cardiology
Year:
2014
Document type:
Article