Effect of IL-15 on the proliferation, differentiation and anti-apoptosis of CD34+ cells in patients with MDS / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 620-623, 2005.
Article
in Zh
| WPRIM
| ID: wpr-356502
Responsible library:
WPRO
ABSTRACT
To study the effect of interleukin-15 (IL-15) on the proliferation, differentiation and apoptosis of MDS CD34(+) cells, CD34(+) cells of high enrichment were separated by MACS system, and cultured in liquid media with different concentration of IL-15 in treated group and without IL-15 in the control group. Apoptosis of hematopoietic precursors was assayed by propidium iodine staining and cell by FCM, and the other MDS CD34(+) cells were stained by cytochemical staining after culture. The results showed that after culture with IL-15 the proliferation and differentiation of MDS CD34(+) cells were obviously promoted. It was found the every lineage of mature cells developed, the expressions of cell surface antigens CD71, CD33 and CD19 all increased in the MDS CD34(+) cell treated with IL-15. It is suggested that IL-15 stimulates the proliferation and differentiation of MDS CD34(+) cells, and partly shows anti-apoptosis effects which may be applicable to the therapy MDS.
Full text:
1
Database:
WPRIM
Main subject:
Pathology
/
Pharmacology
/
Blood
/
Myelodysplastic Syndromes
/
Receptors, Transferrin
/
Bone Marrow Cells
/
Antigens, Differentiation, Myelomonocytic
/
Antigens, CD
/
Cell Cycle
/
Cell Differentiation
Limits:
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2005
Document type:
Article