Proliferation and apoptosis in vitro of umbilical cord blood CD34+CD38- hematopoietic early progenitor cells / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 229-234, 2005.
Article
in Zh
| WPRIM
| ID: wpr-356587
Responsible library:
WPRO
ABSTRACT
To cultivate CD34(+)CD38(-) cells isolated from umbilical cord blood of healthy puerperal women over a longer-period of time for observation of cell division, proliferation, apoptosis, and effects of stem cell factor on the growth of CD34(+)CD38(-) cells, with flow cytometry, CD34(+)CD38(-) cells were isolated from umbilical cord blood of 10 healthy puerperal women and cultivated in stem cell media with supplement of IL-3, IL-6, GM-CSF, EPO, IGF-1 and SCF 6 kinds cell growth stimulating factors for six months. The cell growth curves were established. The effects of stem cell factor on the growth of CD34(+)CD38(-) cells and cell apoptosis were investigated with the single cell gel electrophoresis technique and flow cytometry method, respectively. The results showed that CD34(+)CD38(-) cells isolated from umbilical cord blood were capable of proliferating after being cultivated in vitro over a longer-period of time with no evidence of the presence of excessive apoptosis. In conclusion, under appropriate culture conditions, CD34(+)CD38(-) hematopoietic early progenitor cells from umbilical cord blood can serve as a resource providing a large amount of primitive cells for transplantation therapy after a longer period of cultivation and proliferation in vitro.
Full text:
1
Database:
WPRIM
Main subject:
Hematopoietic Stem Cells
/
Cells, Cultured
/
Apoptosis
/
Antigens, CD34
/
Cell Biology
/
Cell Proliferation
/
Allergy and Immunology
/
ADP-ribosyl Cyclase 1
/
Fetal Blood
/
Flow Cytometry
Limits:
Adult
/
Female
/
Humans
Language:
Zh
Journal:
Journal of Experimental Hematology
Year:
2005
Document type:
Article