Effect of selection techniques on chromosome and ultrastructure of human spermatozoa / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To know the effect of different selection techniques on chromosome abnormality rate and the percentage of spermatozoa with relatively intact head ultrastructure in human sperm.</p><p><b>METHODS</b>The selection techniques of swim-up, Percoll density gradient centrifugation and doule-flection tube were used to observe sperm chromosome abnormality rate, sex chromosomal ratio and the percentage of spermatozoa with relatively intact head ultrastructure.</p><p><b>RESULTS</b>The sperm chromosome abnormality rate, sex chromosomal ratio and the percentage of spermatozoa with relatively intact head ultrastructure remained unchanged after the application of the three selection techniques(P > 0.05).</p><p><b>CONCLUSION</b>The three selection techniques used in this study neither increase the damage to sperm ultrastructure nor exert effects on human chromosome.</p>