Effect of human colon carcinoma-associated fibroblasts on biological behavior of colon carcinoma Lovo cells / 中华胃肠外科杂志
Chinese Journal of Gastrointestinal Surgery
; (12): 759-763, 2013.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-357147
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of human colon carcinoma-associated fibroblasts (CAFs) on proliferation, adhesion, migration and invasion of colon carcinoma Lovo cells.</p><p><b>METHODS</b>The co-culture models among colon CAFs, NFs and Lovo cell were established by conditioned medium (CM) of human colon CAFs and colon normal fibroblasts (NFs). Lovo cells in the blank control group was treated with serum-free culture medium. The effects of human colon CAFs on proliferation, adhesion, migration and invasion of colon carcinoma Lovo cells were detected by cell proliferation assay, adhesion assay, migration assay and Transwell invasion assay.</p><p><b>RESULTS</b>After co-culture with colon CAFs, the absorbance (A) value of Lovo cells was (0.667±0.059) in 48 h and (0.709±0.030) in 72 h. The A value of Lovo cells adhesion to fibronectin was (0.588±0.067). The cell mobility rates were (35.2±8.7)% in 12 h and (64.6±7.1)% in 24 h. The number of invasive cell was (56.2±4.8). All the above parameters were increased compared with those in the blank control group and NFs group (all P<0.01).</p><p><b>CONCLUSION</b>Human colon CAFs can promote the proliferation, adhesion, migration and invasion of colon carcinoma Lovo cells.</p>
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Cell Adhesion
/
Cell Movement
/
Colonic Neoplasms
/
Coculture Techniques
/
Cell Biology
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Cell Line, Tumor
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Cell Proliferation
/
Fibroblasts
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Gastrointestinal Surgery
Year:
2013
Document type:
Article