Preparation of carboxymethyl-chitosan with different molecular weight and its effects on proliferation of skin fibroblasts and keratinocytes / 生物医学工程学杂志

ABSTRACT

Effects of carboxymethyl-chitosan (CM-Chitosan) with different molecular weight on the proliferation of skin fibroblasts and keratinocytes were examined in vitro; bFGF and EGF, as controls, were seperately used for comparison. Chitosan with different molecular weight was prepared by acid degradation and oxidation degradation; CM-Chitosan with different molecular weight was synthesized from corresponding Chitosan. Microscopy and MTT method were applied to evaluate the different effects. The results demonstrated that CM-Chitosan with different molecular weight promoted the proliferation of skin fibroblasts and keratinocytes at 1-1000 ppm, and the concentration at 100 ppm had the strongest effects. The effects of low molecular weight CM-Chitosan were greater than those of high molecular weight CM-Chitosan. CM-Chitosan (Mn= 3KD) had the strongest promotive effects on skin fibroblasts and keratinocytes; it had equivalent effects when compared with bFGF and EGF.