Induction of nucleoside phosphorylase in Enterobacter aerogenes and enzymatic synthesis of adenine arabinoside / 浙江大学学报(英文版)(B辑:生物医学和生物技术)
Journal of Zhejiang University. Science. B
; (12): 520-526, 2008.
Article
in En
| WPRIM
| ID: wpr-359396
Responsible library:
WPRO
ABSTRACT
Nucleoside phosphorylases (NPases) were found to be induced in Enterobacter aerogenes DGO-04, and cytidine and cytidine 5'-monophosphate (CMP) were the best inducers. Five mmol/L to fifteen mmol/L cytidine or CMP could distinctly increase the activities of purine nucleoside phosphorylase (PNPase), uridine phosphorylase (UPase) and thymidine phosphorylase (TPase) when they were added into medium from 0 to 8 h. In the process of enzymatic synthesis of adenine arabinoside from adenine and uracil arabinoside with wet cells of Enterobacter aerogenes DGO-04 induced by cytidine or CMP, the reaction time could be shortened from 36 to 6 h. After enzymatic reaction the activity of NPase in the cells induced remained higher than that in the cells uninduced.
Full text:
1
Database:
WPRIM
Main subject:
Pentosyltransferases
/
Pharmacology
/
Vidarabine
/
Enzyme Induction
/
Enterobacter aerogenes
/
Cytidine
/
Cytidine Monophosphate
Language:
En
Journal:
Journal of Zhejiang University. Science. B
Year:
2008
Document type:
Article