Imperatorin Suppresses Degranulation and Eicosanoid Generation in Activated Bone Marrow-Derived Mast Cells
Biomolecules & Therapeutics
; : 421-427, 2015.
Article
in English
| WPRIM (Western Pacific)
| ID: wpr-36718
Responsible library:
WPRO
ABSTRACT
Imperatorin has been known to exert many biological functions including anti-inflammatory activity. In this study, we investigated the inhibitory effects of imperatorin on the production of inflammatory mediators in mouse bone marrow-derived mast cells (BMMC). Imperatorin inhibited degranulation and the generation of eicosanoids (leukotriene C4 (LTC4) and prostaglandin D2 (PGD2)) in IgE/antigen (Ag)-stimulated BMMC. To elucidate the molecular mechanism involved in this process, we investigated the effect of imperatorin on intracellular signaling in BMMC. Biochemical analyses of the IgE/Ag-mediated signaling pathway demonstrated that imperatorin dramatically attenuated degranulation and the production of 5-lipoxygenase-dependent LTC4 and cyclooxygenase-2-dependent PGD2 through the inhibition of intracellular calcium influx/phospholipase Cgamma1, cytosolic phospholipase A2/mitogen-activated protein kinases and/or nuclear factor-kappaB pathways in BMMC. These results suggest that the effects of imperatorin on inhibition of degranulation and eicosanoid generation through the suppression of multiple steps of IgE/Ag-mediated signaling pathways would be beneficial for the prevention of allergic inflammation.
Full text:
Available
Database:
WPRIM (Western Pacific)
Main subject:
Phospholipases
/
Protein Kinases
/
Prostaglandin D2
/
Eicosanoids
/
Calcium
/
Leukotriene C4
/
Mitogen-Activated Protein Kinases
/
Cytosol
/
Inflammation
/
Mast Cells
Limits:
Animals
Language:
English
Journal:
Biomolecules & Therapeutics
Year:
2015
Document type:
Article