Effect of rhDCN on the expression of HLA molecules on HepG2 cell lines / 肿瘤研究与临床
Cancer Research and Clinic
; (6): 298-300, 2009.
Article
in Zh
| WPRIM
| ID: wpr-380968
Responsible library:
WPRO
ABSTRACT
Objective To observe the regulatory effect of recombinant human DCN on the expression of HLA class Ⅰ, Ⅱ molecule on hepatoma carcinoma cell HepG2 and investigate the relatively immune mechanism of human DCN on enhancing anti-tumor effect. Methods After transfected HepG2 cells with pcDNA3.1 (+)-DCN by liposome transfection, the expression of HLA class Ⅰ , Ⅱ molecule and the apoptotic indexes were analyzed by the flow cytometer. Results The expression of HLA class Ⅰ , Ⅱ molecule on the HepG2 cells transfected with pcDNA3.1 (+)-DCN was up-regulated with the apoptosis indexes being significantly higher than that of other control groups. Conclusion The human DCN can induce HepG2 apoptosis and up-regulate the expressions of HLA class Ⅰ , Ⅱ molecule which may contribute to its antitumor effect.
Full text:
1
Database:
WPRIM
Language:
Zh
Journal:
Cancer Research and Clinic
Year:
2009
Document type:
Article