Display of heterologous proteins on the surface of E. coli by Red recombineering / 中华微生物学和免疫学杂志
Chinese Journal of Microbiology and Immunology
; (12): 656-660, 2008.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-382102
Responsible library:
WPRO
ABSTRACT
Objective To display of heterologous proteins on the surface of E. coli . Methods The 1653 bp luciferase report gene was knocked in Ipp and ompA genes of chromosome by Red recombine system and selection-counter selection kan/sacB. Results The quantitative analysis results of exogenous lu-ciferase expression displayed that it could be expressed as fusion with the outer membrane proteins on the cell surface. The fusion protein of foreign protein and outer membrane protein Lpp-OmpA-Luc could be high-effi-ciently displayed on cell surface. Conclusion The stable expression of exogenous gene on the surface of E. coli had no effect on the bacterial growth and propagation.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Microbiology and Immunology
Year:
2008
Document type:
Article