Detection of intracellular calcium concentration and apoptosis in Thapsigargin-induced human colon cell line SW480 / 中国医师杂志
Journal of Chinese Physician
; (12): 334-335, 2009.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-395523
Responsible library:
WPRO
ABSTRACT
Objective To investigate the mechanism of Thapsigargin in the proliferation of SW480 and provide experimental basis for development of anti-signaling therapy for patients with colon cancer by detection of intracellular calcium concentration in Thapsigargininduced human colon cell line SW480,.Methods Level of[Ca2+]was measured by fluorescence measurement with fura-2/AM,a fluorescent index of calcium.The suppression rate of SW480 cells was calculated by MTY assay.Results Thapsigargin promoted the levels of [Ca2+]from(133.26±5.17,197.46±4.62)to(436.63±5.21,766.41±3.12),and increased the suppression rate from(10.67±5.28)to(31.46±7.21).When W7 Was combined used with Thapsigargin,no significant changes were found in the levels of[Ca2+]and increment of the suppression rate.Condusion Thapsigargin might promote the proliferation of SW480 cells and lead to apoptosis.but the signaling pathway still remain to he studied.
Full text:
Available
Database:
WPRIM (Western Pacific)
Type of study:
Diagnostic study
Language:
Chinese
Journal:
Journal of Chinese Physician
Year:
2009
Document type:
Article