Plant vector construction and expression of Der f1 allergen of Dermatophagoides pteronyssinu / 中国免疫学杂志
Chinese Journal of Immunology
; (12): 250-253, 2010.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-403227
Responsible library:
WPRO
ABSTRACT
Objective:
To construct the plant expression vector of Der f1 allergen of Dermatophagoides pteronyssinu and expression in tobacco lamina.Methods:
The Der f1 gene was amplified from the glycerin bacterium which contained pET28a(+)-Der f1 plasmid,cloned into the pMD 19-T plasmid,and then sequenced.The Der f1 gene was digested by ClaⅠand SalⅠ,and cloned into potato virus X (PVX) to construct plant expression vector PVX-Der f1,and then was transformed agrobacterium tumefaciens.The positive one was selected to infect tobacco lamina for expressing target protein.The protein was identified and analysed by SDS-PAGEand Western blot.Results:
Digestion and sequence analysis confirmed that the plant expression vector was correct,and the SDS-PAGE and Western blot results showed that the molecular weight of the protein was about 34M_r and it could specific binding with positive serum.Conclusion:
The plant expression vector of Der f1 is successfully constructed and the recombinant protein is also produced.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Immunology
Year:
2010
Document type:
Article