δ-opioid receptors protect neurons against neuronal injury induced by oxygen-glucose deprivation / 复旦学报(医学版)
Fudan University Journal of Medical Sciences
; (6): 389-393, 2009.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-405741
Responsible library:
WPRO
ABSTRACT
Objective To investigate the effect of cortical 8-opioid receptor (DOR) on oxygen-glucose deprivation-induced (OGD-induced) neuronal injury. Methods Primary cultured cortical neurons incubated with selective DOR agonist (TAN-67) and antagonist (naltrindole) or PKC inhibitor (chelerythrine, CHE) were exposed to OGD. Lactate dehydrogenase (LDH) release was detected after 24 h reperfusion. The expression levels of DOR were measured by Western blot. Results Compared with OGD group, TAN-67 significantly decreased OGD-indueed LDH release, and increased the expression levels of DOR, while nahrindole aggravated neuronal injury and decreased the DOR protein expression. CHE could abolish the LDH down-regulation induced by TAN-67 plus OGD (P< 0.05, compared with TAN-67 treated group). Conclusions DOR activation protects neurons against OGD injury. PKC might take part in the neuroprotection pathways of DOR.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Fudan University Journal of Medical Sciences
Year:
2009
Document type:
Article