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Influencing factors in the refolding process of artificial molecular chaperone assisting chicken IL-18 recombination protein / 中国兽医学报
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-406341
Responsible library: WPRO
ABSTRACT
The recombinant plasmid of mChlL-18 prokaryotic expression was transformed into E.coli BL21(DE3) strain and then induced by IPTG at 37℃.After crushed and washed,the expressing inclusion bodies were thoroughly denatured with 6 mol/L guanidine hydrochloride.Then according to experiment design,the effects of rChlL-18 protein refolding yield at different densities were investigated by the systems of artificial chapercne at different densities.Experiment results indicate that there is a optimal condition on assiting rChIL-18 protein by using the artificial chaperone technique.The optimal condition can improve the refolding yield of rChIL-18 protein,and then the expressed product of fusion chicken IL-18 gene in E.coli has a relativity high bioactivity.

Full text: Available Database: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Veterinary Science Year: 2009 Document type: Article
Full text: Available Database: WPRIM (Western Pacific) Language: Chinese Journal: Chinese Journal of Veterinary Science Year: 2009 Document type: Article
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