Determination of Baicalin and Berberine Hydrochloride in Compound Qinbo Granules by HPLC / 中药新药与临床药理
Traditional Chinese Drug Research & Clinical Pharmacology
; (6): 349-351, 2009.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-406471
Responsible library:
WPRO
ABSTRACT
Objective To establish a HPLC method for the determination of baicalin and berberine hydrochloride in Com-pound Qinbo Granules. Methods The gradient elution was performed on a Diamonsil C18 column using method-water (0. 05 % phosphate acid and 0. 1% trithylamine) as mobile phase, and the detection wavelength was 276 nm. Results The linear calibration curve of baicalin was obtained in the range of 10. 08~201.6 mg·L-1 with an average recovery of 98. 1% (RSD=1.11%). The linear calibration curve of berberine hydrochloride was in the range of 4. 08~81.6 mg·L-1 with an average re-covery of 97.9 % (RSD=1.24 %). Conclusion The method is simple and reproducible. It can be used for the quality con-trol of Compound Qinbo Granules.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Traditional Chinese Drug Research & Clinical Pharmacology
Year:
2009
Document type:
Article