Effect of naoningkang granules on the CD62P level of platelet in rats with cerebral ischemic preconditioning and cerebral ischemia-reperfusion / 中国组织工程研究

ABSTRACT

BACKGROUND: As molecular marker of platelet, CD62P not only reflects activated degree and functional status of platelet but also mediates adherent function of platelet with neutrophilic granulocyte and endothelial cell so as to deteriorate reperfusion injury after cerebral ishcemia.OBJECTIVE: To observe effect of naoningkang (NNK) granules on CD62P level of platelet in rats with cerebral ischemic preconditioning and cerebral ischemia-reperfusion and compare with aspirin at the same time.DESIGN: Randomized control study.SETTING: Encephalopathy Center of Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine.MATERIALS: The experiment was completed in Shandong University from July 1998 to February 1999. Totally 48 male Wistar males were selected from Experimental Animal Center of Shandong University. All rats were randomly divided into model group, sham operation group, aspirin group and NNK group with 12 in each group. Six rats were selected from each group for modeling with cerebral ischemic preconditioning method, and other 6 with ischemia-reperfusion method. NNK granules were composed of chuanxiong, flea and seaweed, and contained 5 g/g dried medicinal herbs.METHODS: Ischemic preconditioning (IPC) model and cerebral ischemiareperfusion model were made directed according to Pulsinelli four tubes method and Liu's method respectively. Blood of rats were obtained under anesthesia after 24-hour ischemia-reperfusion. CD62P level on the membrane surface of platelet in rats with cerebral ischemic preconditioning and cerebral ischemia-reperfusion was assayed and compared with fluorescence antibody labelling method on flow cytometer.MAIN OUTCOME MEASURES: CD62P level of platelet in rats with cerebral ischemic preconditioning and cerebral ischemia-reperfusion.chemia-reperfusion, CD62P level of rats in the two model groups was increased obviously (P ＜ 0.01), but the increase in NNK group and aspirin mong cerebral ischemic preconditioning models showed less changes in the two kinds of models, both NNK and aspirin showed positive effect on CD62P, and the effect of NNK group was a little superior to that of aspirin group, but the difference was not significant (P ＞ 0.05).CONCLUSION: NNK has positive effect on CD62P expression in both cerebral ischemic preconditioning and cerebral ischemia-reperfusion models, and the effect of NNK group is a little superior to that of aspirin group,this suggests that NNK can inhibit its aggregation, improve local microcirculation of brain through interfering in activation of platelet so as to protect cerebral neurons.