Cloning of Mouse Enamel Matrix Serine Proteinase Encoding Mature Protein / 吉林大学学报(医学版)
Journal of Jilin University(Medicine Edition)
; (6): 705-709, 2003.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-410103
Responsible library:
WPRO
ABSTRACT
Objective:
To clone cDNA of enamel matrix serine proteinase (EMSP1) encoding mature protein from mouse dental germs.Methods:
Total RNA was isolated from developing incisors and molars of 7 days mouse pups and reverse-transcribed into cDNA. Two pairs of specific primers was designed to obtain the desired gene by Touchdown PCR and Nested PCR. The segment was inserted into Vector pMD-18T, and recombined vectors was transformed into E.coli JM109.The positive clone was chose and analysed by restriction endonuclease mapping and DNA sequencing.Results:
700 bp of cDNA of mouse EMSP1 was sueccessfully cloned from mouse tooth germs tissue. The sequence was consistent with that displayed in PubMed.Conclusion:
The mouse EMSP1 cDNA encoding mature protein is obtained for further study.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Jilin University(Medicine Edition)
Year:
2003
Document type:
Article