Protective effect of cardiomyopeptidin on cultured myocardial cells injured by anoxia-reoxygenation / 中国病理生理杂志
Chinese Journal of Pathophysiology
; (12): 481-484, 2001.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-410302
Responsible library:
WPRO
ABSTRACT
AIM:
To study the protective effect of cardiomyopeptidin (CMP) attributed to polypeptide on cultured myocardial cells injured by anoxia-reoxygenation.METHODS:
The anoxia-reoxygenation injury model were developed, anoxia for 60 min and reoxygenation for 30 min. The effect of CMP on myocardial ultrastructure was observed. [Ca2+]i was estimated with adherent cell analysis and sorting 570(ACAS 570) laser cytometer and measured with fluorescent dye Fura-2-AM, the lipid fluidity of cellular membrane was determined by fluorescence polarization technique.RESULTS:
CMP could obviously improve the ultrastructure of myocardial cells and dose-dependently decrease [Ca2+]i and increase the lipid fluidity of cellular membrane, CMP also could markedly reduce the chromaticity value of pseudo-colour graphic model of Ca2+.CONCLUSION:
Cardiomyopeptidin has an obvious protective effect on cultured myocardial cells injured by anoxia-reoxygenation, this may be related to its effect of decreasing [Ca2+]i and increasing lipid fluidity of cellular membrane.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Pathophysiology
Year:
2001
Document type:
Article