Cloning and expression of polycystin-1 intracellular region cDNA / 第二军医大学学报
Academic Journal of Second Military Medical University
; (12): 313-315, 2001.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-410495
Responsible library:
WPRO
ABSTRACT
Objective:
To obtain polycystin-1 intracellular region.Methods:
cDNA of polycystin-1 intracellular region was generated by PCR and then cloned into pProEX Hta, which was prokaryotic expression vector. After verified by sequencing, the recombinant was transformed into E.coli host to express and purify the fusion protein by affinity chromatography.Results:
660 bp cDNA of polycystin-1 intracellular region and 2.6×104 fusion protein were obtained.Conclusion:
The fusion protein containing polycystin-1 intracellular region is obtained and is helpful for preparing anti-polycystin-1 monoclonal antibody.
Full text:
Available
Database:
WPRIM (Western Pacific)
Type of study:
Health technology assessment
Language:
Chinese
Journal:
Academic Journal of Second Military Medical University
Year:
2001
Document type:
Article
