A method of on-column sample stacking in capillary electrophoretic analysis of micro-scale amino acids / 中国生化药物杂志
Chinese Journal of Biochemical Pharmaceutics
; (6): 71-73, 2001.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-411424
Responsible library:
WPRO
ABSTRACT
Purpose The aim is to develop a method for the analysis of micro-scale amino acids in biological samples.Methods After having been extracted and dried,the residues were dissolved in a running buffer or a distilled water and acetonitrile.The samples were loaded into column for 5,25 or 50s at a hydrodynamic injection and were separated by capillary electrophoresis.The effect of different dissolvent on the sample stacking was observed.Results A 100-fold improvement in the amount of material that can be injected into a capillary column without loss of resolution was shown.Conclusion The marked effect of sample stacking was achieved by the using distilled water and acetonitrile(1∶1,v/v) as dissolvent.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Biochemical Pharmaceutics
Year:
2001
Document type:
Article