Effects of Dexamethasone on Secretion of Apolipoproteins A Ⅰ ,AⅡ, B100, C Ⅲ and E by Cultured HepG2 Cells / 生物化学与生物物理进展
Progress in Biochemistry and Biophysics
; (12): 77-80, 2001.
Article
in Chinese
| WPRIM (Western Pacific)
| ID: wpr-411766
Responsible library:
WPRO
ABSTRACT
In order to obersve the effect of dexamethasone on the secretion of apolipoproteins A Ⅰ, A Ⅱ, C Ⅲ, B100 and E by cultured HepG2 cells.The apolipoprotiens contents in culture media were measured by radioimmuodiffusion assay (RID) kits developed by authors' research unit. 20-fold lyophilizely condensed culture media were used for the assays. The results showed that dexamethasone can increase the secretion of apoC and E, and inhibit the secretion of apoAⅡ , B100 and CⅢ; and the effect of dexamethasone were strengthened in a dose-dependent manner. When the concentration of dexamethasone was 5.5 × 10-5mol/L in the culture media, the secretion of apolipoprotein A Ⅰ and E increased 36.6% and 49.4%(P < 0.01) respectively, while the secretion of apo AⅡ , B100 and CⅢ decreased 38.9%、 31.9% and 29.8% (P<0.01) respectively.
Full text:
Available
Database:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Progress in Biochemistry and Biophysics
Year:
2001
Document type:
Article